PSI-14 Effects of Estrus Expression on the Microbial Community Composition of the Uterus in Beef Cows on day 15 of the Estrous Cycle

Abstract The objective of this study was to evaluate the effects estrus expression on uterine microbiota composition cows day 15 estrous cycle. Non-pregnant Bos taurus beef (n = 23) were exposed a pre-synchronization step followed by 7-d CO-Synch synchronization protocol. On days -10, -3,0 (exogenously induced ovulation),7 and 14, transrectal ultrasonography performed ovarian structures, ensure synchrony, determine side ovulation. Estrotect patches applied -3, with an activated patch (rubbed > 50%) 0 considered have displayed heat. Cows harvested individual swabs collected from each horn using aseptic techniques. DNA extracted entire (V1-V9 hypervariable regions)16s rRNA gene sequenced. Sequences analyzed QIIME2 Pipeline. Burkholderiaceae, Chitinophagaceae, Rikenellaceae, Burkholderia, Rikenellaceae RC9 gut group, Lachnospiraceae AC2044 Vibrionimonas, Prevotellaceae UCG-001, dgA-11 group abundances differed significantly (P < 0.05) in that exhibited compared non-estrual cows. In conclusion, there significant differences microbial community between